GOSH-UCL CRISPR Centre

Our Core Services:

• Knockout (KO) – Targeted gene disruption via CRISPR-induced indels (£3,000 per line)
• Knock-in (KI) – Point Mutation – Precise single nucleotide variant introduction (£4,000 per line)
• Compound Heterozygote Editing – Custom CRISPR strategies introducing multiple mutations (£5,000 per line)

Additional Services (Based on Feasibility & Capacity):

• Non-DSB Gene Editing – Advanced editing techniques for precise modifications without double-strand breaks (DSBs).
• Gene Regulation & Tagging – CRISPRi, CRISPRa, Safe harbour gene expression, fluorescent/epitope/degron tagging.
• Conditional & Large-Scale Modifications – Conditional knockouts, large deletions, and CRISPR screens.
• Difficult-to-Edit Projects – Customised approaches for complex modifications.
• High-Throughput CRISPR Genotyping – Efficient sequencing and validation of edited clones.
• Training & Consultation – Hands-on guidance for CRISPR applications and experimental design.

The CRISPR Centre is open to GOSH and UCL researchers, as well as external collaborators where capacity allows.

How to Request Services

To discuss project feasibility or request CRISPR services, please get in touch with our team at CRISPRCentre@ucl.ac.uk.

For more information, please reach out to

Azeezat Aminu – azeezat.aminu@gosh.nhs.uk

Prof. Sergi Castellano – s.castellano@ucl.ac.uk

Dr. Shalini Reddy – shalini.reddy@ucl.ac.uk

Prof. Manju Kurian – Manju.Kurian@ucl.ac.uk